TALDO1 Knockout cell line (HT-29)

TALDO1 Knockout cell line (HT-29) Order

Catalog#

YKO-H1184

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

TALDO1 Knockout cell line (HT-29)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

TALDO1

Gene ID

6888

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%McCOY's 5A+10% FBS

Target region

Knockout region: Exon 2 (based on transcript TALDO1-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HT-29			STR Info (Cell bank) Cell Line:HT-29
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	11	12		11	12
D7S820	10	10		10	10
D16S539	11	12		11	12
VWA	17	19		17	19
TH01	6	9		6	9
AMEL	X	X		X	X
TPOX	8	9		8	9
CSF1PO	11	12		11	12
D12S391	18.3	21
FGA	20	22
D2S1338	19	23
D21S11	29	30
D18S51	13	13
D8S1179	10	10
D3S1358	15	17
D6S1043	12	14
PENTAE	14	16
D19S433	14	14.2
PENTAD	11	13
D1S1656	15	16

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TALDO1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HT-29 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TALDO1 gene knockout TALDO1 Knockout cell line (HT-29) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

TALDO1 Knockout cell line (HT-29)

Catalog#

YKO-H1184

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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